Date of Award
MS in Biology
Wan S Yang
Cell death is a biological process that is essential in proper cellular homeostasis, development, and function. Malfunction of this system can lead to different diseases and cancers. Ferroptosis is a form of cell death that occurs as a result of the accumulation of lipid peroxides, that are dependent on intracellular levels of iron. Iron is essential to multiple metabolic processes in the cell, including the catalyzation of lipid peroxides. Thus, intracellular iron levels must be tightly regulated. Our goal is to create a cellular model to study and detect changes in intracellular iron levels. Through the use of a genetically encoded fluorescent iron sensor, we have generated a stable cell line that provides fluorescent readout on intracellular iron levels. This model can be used to screen for genes, small molecules, and drugs that affect and regulate intracellular iron levels. These findings can then be used to discover new regulators of ferroptosis, data that could be essential to the treatment of ferroptosis-linked diseases.
Rahman, Daoud F., "GENETICALLY ENCODED IRON SENSOR CELL LINE: A MODEL FOR STUDYING FERROPTOSIS AND CELLULAR IRON METABOLISM" (2023). Theses and Dissertations. 593.